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Modern Water RaPID Assay Total BTEX / TPH 100 Tube Kit - A00162
Item #:
A00162
Brand:
Modern Water
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A00162 RaPID Assay Total BTEX / TPH 100 Tube Kit by Modern Water
RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.
Features:
- Rapid field testing procedure for analysis of soil and water samples
- Quantitative data results with excellent analytical precision
- Results available in approximately 60 minutes
- Training available
- Magnetic particle immunoassay
- Correlated to TPH methods (knowledge of fuel source required)
Test result type
- Quantitative, semi-quantitative or qualitative
Samples per kit
- 100 test kit (tests up to 80+ samples) (Five kit minimum order)
Assay range
- Soil: 0.9 ppm to 30 ppm as total BTEX standard
- Water: 0.02 to 3.0 ppm as total BTEX standard
- asTPH:range varies based on fuel source
Sample preparation
- Soil samples require prior extraction using the sample extraction kit (sold separately).
- The sample extraction kit provides materials for 12 soil sample extractions with methanol.
Sampling time
- Soil extraction time is typically two minutes per sample plus assay run time of approximately 60 minutes.
Specificity
The total BTEX/TPH concentrations of the indicated contaminant types in soil samples are expressed below, at each ofthe three kit calibrator levels, in units comparable to results from GC method 8015 (TPH).
The total BTEX/ TPH RaPID assay immunoassay test does not differentiate between BTEX and other petroleum hydrocarbons.The table below shows compounds at the limit of quantitation (LOQ) - an approximate concentration required to yield a positive result at the lowest standard. The IC50 is the concentration required to inhibit one-half of the colour produced by the negative control. It is also used to calculate cross-reactivity values to similar compounds.
Test kit components
- Antibody coated magnetic particles for analysis of 100 test tubes
- Zero standard, wash, enzyme conjugate, colour development and stop reagents
- Standards for 0.09, 0.35 and 3.0 ppm as total BTEX
- Kit control as 2.1 ppm as total BTEX
- Disposable test tubes
Storage & precautions
- Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
- Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
- Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
- Kits must be brought to 64° to 81°F (18° to 27°C) before use
- Do not expose colour solution to direct sunlight
Required test materials
- Total BTEX/TPH 100 tube kit (A00162)
- Sample extraction kit(for soil samples only) (A00165EB)
- Total BTEX sample diluent 100ml (as needed to increase range)
Required test equipment
- RaPID Assay accessory kit which contains (6050100 (P)/6997010 (R)): RPA-II RaPID analyser (6000111), Magnetic separation rack (A00004), Repeator pipet (A00008), Adjustable volume pipet (A00176), Vortex mixer (A00014), Portable balance (A00131), Digital timer (A00015)
- Repeator pipet tips (A00009)
- Adjustable pipet tips (A00013)
Other recommended materials
- Latex gloves
- Liquid and solid waste containers
- Calculator
- Absorbant paper for blotting
- Marking pen
Contaminant | Std. 1 equiv. | Std. 2 equiv. | Std. 3 equiv. |
Gasoline | 10 | 50 | 500 |
Jet A | 200 | 500 | 2500 |
JP-4 | 20 | 80 | 750 |
JP-5 | 40 | 100 | 500 |
Turbine (Jet) Fuel | 10 | 50 | 1000 |
Kerosene | 50 | 150 | 1250 |
Artic Diesel | 3 | 10 | 60 |
Diesel Fuel | 10 | 40 | 300 |
Home Heating Oil | 20 | 70 | 600 |
Fuel Oils | 10 | 50 | 500 |
Contaminant | LOQ | IC50 |
BTEX* | 0.9 | 6.55 |
m-Xylene | 2.5 | 18.0 |
p-Xylene | 4.3 | 31.0 |
o-Xylene | 6.5 | 47.0 |
Ethylbenzene | 10.8 | 78.0 |
Toluene | 10.2 | 74.0 |
Benzene | 70.6 | 510 |
Naphthalene | 0.82 | 5.9 |
1,2,4 -Trimethylbenzene | 1.6 | 11.5 |
Anthracene | 38.8 | 280 |
Styrene | 3.6 | 26 |
Phenanthrene | 2.2 | 16.0 |
Creosote | 6.6 | 47.6 |
1,3,5 -Trimethylbenzene | 4.8 | 35.0 |
Acenapthene | 85.2 | 620 |
n-Propylbenzene | 6.5 | 47.0 |
n-Heptane | 99.7 | 720 |
n-Hexane | >138 | >1000 |
n-Octane | >138 | >1000 |
n-Nonane | >138 | >1000 |
Cyclohexane | >138 | >1000 |
n-Decane | >138 | >1000 |
Methylene Chloride | >1000 | >1000 |
Trichloroethylene | >1000 | >1000 |