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Modern Water Atrazine 100 Test Tube Kit - A00071
Item #:
A00071
Brand:
Modern Water
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A00071 Atrazine 100 Test Tube Kit by Modern Water
RaPID Assay® is a rapid field or laboratory enzyme immunoassay method for the analysis of soil and water for remediation, assessment, and industrial testing.
Features:
- Rapid field testing procedure for analysis of soil, food or water samples
- Quantitative data results with excellent analytical precision
- Results available in approximately 60 minutes
- Tests up to 50 samples at once
- Magnetic particle immunoassay
- Training available
- EPA SW-846 method #4670 for drinking water
Test result type
- Quantitative, semi-quantitative or qualitative depending on protocol selected
Samples per kit
- 100 test kit (tests up to 80+ samples)
Assay range
- 0.04 to 5.0 ppb
- LDD = 0.1 ppb
Sample methods
- Methods exist for soil and a wide variety of commodities
Sampling time
- 'Sample-to-Data' in approximately 60 minutes
Specificity
The proprietary antibody used in this assay is specific for atrazine. The cross-reactivity of the Atrazine RaPID Assay® for various triazine analogues can be expressed as least detectable dose (LDD) which is estimated at 90% B/B0 or as the concentration estimated (inhibitory concentration) at 50% B/B0 (IC50).
Basic Test Procedure
- Add 200 μL of calibrator or prepared sample, 250μL enzyme conjugate and 500 μL antibody coupled magnetic particles.Vortex
- Incubate for 15 minutes
- Using the RaPID magnetic separator, decant and wash
- Add 500 μL colour solution
- Incubate for 20 minutes
- Stop the reaction and read colour at 450 nm
- Quantitive results and QC parameters are calculated and printed automatically using the RPA-II® spectrophotometer
Test kit components
- Antibody coated magnetic particles, enzyme conjugate, colour development, stop andwash reagents for analysis of 100 test tubes
- Standards for 0.1 1.0 and 5.0 ppb Atrazine
- Zero standard and 3.0 ppb control standard
- Disposable test tubes
- Test kit instructions
Storage & precautions
- Shelf life is typically one year from date of manufacture, with specific kit expiration date information provided on product packaging
- Reagents must be stored at 39° to 46°F (4° to 8°C) when not in use
- Store at ambient temperature 64° to 81°F (18° to 27°C) is acceptable for day of use
- Kits must be brought to 64° to 81°F (18° to 27°C) before use
- Do not expose colour solution to direct sunlight
Required test materials
- Atrazine RaPID Assay (A00071)
- Atrazine sample diluent (100mL) (A00051)
Required test equipment
- Accessory kit (purchase) (6050100)
- Accessory kit (rental) (6997010)
- Accessory kit components: Magnetic separation rack (A00004), Repeator pipette (A00008), Adjustable volume pipette (A00176), Adjustable volume pipette (6000111), RPA-II analyser with printer (A00014), Vortex mixer (A00014), Digital timer (A00015)
Contaminant | LDD | IC50 |
Atrazine | 0.046 | 0.72 |
Ametryn | 0.053 | 0.39 |
Propazine | 0.033 | 0.74 |
Prometryn | 0.054 | 0.64 |
Prometon | 0.056 | 2.22 |
Simazine | 0.340 | 4.90 |
Terbutryn | 0.090 | 5.50 |
Terbutylazine | 0.310 | 15.5 |
Desethyl Atrazine | 0.062 | 3.21 |
Cyanazine | 1.000 | >10,000 |
6-Hydroxy Atrazine | 1.100 | 148 |
Desisopropyl Atrazine | 0.800 | 217 |